Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Corydalis saxicola Bunting Total Alkaloids ameliorate diet-induced non-alcoholic steatohepatitis by regulating hepatic PI3K/Akt and TLR4/NF-κB pathways in mice.

doi: 10.1016/j.biopha.2022.113132

Figure Lengend Snippet: Fig. 3. CSBTA reduced intrahepatocellular lipid accumulation and improved hepatocyte survival under high fat condition. (A) Effects of CSBTA on hepatic TG and TC of HFHC-fed mice (n = 5). (B) The serum levels of IL-1β and TNF-α of mice were tested after CSBTA treatment (n = 5). (C) Expression levels of genes involved in de novo lipogenesis and inflammation measured by qPCR (n = 6). (D) p-PI3K (p85)/PI3K (p85) and p-Akt/Akt were detected by Western blot (n = 3). (E) TLR4 /GAPDH and p-NFκB (p65)/NFκB (p65) were detected by Western blot (n = 3). Data were shown as mean ± SD, statistical analyses were performed by one-way ANOVA followed by Bonferroni post hoc test; #p < 0.05, ##p < 0.01 vs. the NC group. *p < 0.05, **p < 0.01 vs. the HFHC group.

Article Snippet: Thermal cycling and fluorescence detection were performed with Hieff UNICON® Power qPCR SYBR Green Master Mix (Yeasen, Shanghai, China).

Techniques: Expressing, Western Blot

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Corydalis saxicola Bunting Total Alkaloids ameliorate diet-induced non-alcoholic steatohepatitis by regulating hepatic PI3K/Akt and TLR4/NF-κB pathways in mice.

doi: 10.1016/j.biopha.2022.113132

Figure Lengend Snippet: Fig. 4. CSBTA relieved hepatocellular lipotoxicity induced by palmitate. (A) Viability of HepG2 cells treated by different concentrations of CSBTA (n = 5). (B) Representative Oil Red O staining in HepG2 cells after the addition of palmitic acid (n = 5). Scale bar = 20 µm. (C, D) Relative TC and TG levels in HepG2 cells (n = 5). (E) Expression levels of genes in HepG2 cells involved in lipogenesis and inflammation measured by qPCR (n = 5). (F) p-PI3K (p85)/PI3K (p85) and p-Akt/ Akt in HepG2 cell extracts were detected by pcr(n = 3). Data were shown as mean ± SD, statistical analyses were performed by one-way ANOVA followed by Bonferroni post hoc test; #p < 0.05, ##p < 0.01 vs. the Vehicle group. *p < 0.05, **p < 0.01 vs. the PA group.

Article Snippet: Thermal cycling and fluorescence detection were performed with Hieff UNICON® Power qPCR SYBR Green Master Mix (Yeasen, Shanghai, China).

Techniques: Staining, Expressing